乙醇提取物对斜纹夜蛾细胞Sl-1的细胞毒性比较

Cytotoxicity of ethanol extracts on Spodoptera litura (Sl-1) cell line

用MTT法测定20种泰国植物乙醇提取物对斜纹夜蛾(Spodoptera litura)卵巢细胞系Sl-1的增殖抑制作用,用倒置相差显微镜观察细胞形态变化,用流式细胞术测定细胞线粒体膜电位变化。结果表明:5种植物(姜(Zingiber officinale Roscoe)、高良姜(Alpinia galangl(L.)Willd.)、印楝泰国变种(Azadirachta indica A.Juss var.siamensis Valeton)、姜黄(Curcuma longa Dennst)、泰国青柠(Citrus hystrix DC))乙醇提取物的细胞增殖抑制效果较好,抑制率明显大于阳性对照鱼藤酮,并且与提取物浓度成剂量依赖关系。不同提取物处理24h细胞形态发生明显改变,包括细胞贴壁性减弱、空泡化、细胞碎片产生;48h后细胞形态变化加强、空泡化严重、细胞碎片增多。5种植物提取物对细胞线粒体膜电位均有影响,其中印楝泰国变种、泰国青柠效果最好。初步认为姜、高良姜、印楝泰国变种、姜黄、泰国青柠对Sl-1有细胞毒性作用,值得进一步研究。

The inhibition of cell proliferation,cell morphological change and mitochondrial membrane potential change of ethanol extracts of 20 Thailand plants on Spodoptera litura(Sl-1)cell were investigated by MTT test,inverted phase contrast microscope and flow cytometry,respectively.The results showed that the Sl-1cell proliferation inhibition effect of 5ethanol extracts including Thailand plants(Zingiber officinale Roscoe,Alpinia galangl(L.)Willd.,Azadirachta indica A.Juss var.siamensis Valeton,Curcuma longa Dennst and Citrus hystrix DC)was better than that of the control rotenone,and the inhibition was dose-dependent.After 24 hof the treatment,cells showed obvious morphological changes,including adhesion capacity reduction,vacuole and debris appearance.After 48 hof the treatment,more and more vacuoles and debris were present obviously in the media.Moreover,these five ethanol extracts could induce cell mitochondrial membrane potential change,among which A.indica A.Juss var.siamensis Valeton and C.hystrix DC were more effective.In conclusion,Z.officinale Roscoe,A.galangl(L.)Willd.,A.indica A.Juss var.siamensis Valeton,C.longa Dennst and C.hystrix DC possessed cytotoxicity on Sl-1cells and deserved further researches.