粘绿木霉Gv29-8的碳水化合物活性酶类蛋白预测及遗传关系分析

Prediction and genetic relationship of CAZymes proteins from Trichoderma virens Gv29-8 genome

利用CAT(CAZymes analysis toolkit)在线分析程序对Trichoderma virens Gv29-8中碳水化合物活性酶类蛋白(carbohydrate-active enzymes,CAZymes)不同亚家族的分布情况进行分析,结果表明:粘绿木霉Gv29-8中含有185个CAZymes蛋白,分为主要类别和复合类别两种类型;其中,主要类别含有71个GH蛋白、54个CBM蛋白及12个AA蛋白、25个CE蛋白、3个PL蛋白、3个GT蛋白;复合类型含有17个蛋白。遗传关系分析表明,CAZymes分类应进一步细化到更小的类别。

Carbohydrate active enzymes protein(CAZymes),one large kind of important proteins of plant pathogenic fungi and bacteria,plays an important role in its growth and development.Trichoderma virens Gv29-8 is the important bio-control agent of Trichoderma ssp.,inhibiting many plant pathogens.Predicting and analyzing the characteristics of the CAZymes is of great significance.Based on 377 secreted proteins obtained preliminary data,CAZymes protein was predicted with CAT forecasting procedures.185 CAZymes proteins were found in T.virens Gv29-8 and divided into major categories and composite category.The former includes 71 GHs,54 CBMs,12 AAs,25 CEs,3 PLs and 3 GTs;the latter includes 17 CAZymes proteins.Genetic analyses showed that CAZymes classification should be further refined to a smaller category.It will lay a solid theoretical foundation for further studying its function.