摘要
目的 建立灵敏度高、选择性好、试剂稳定的血清钙测定方法。方法 在pH9.6的2氨基-2-甲基-1,3-丙二醇(AMP)缓冲介质中,以偶氮氯膦I作显色剂,8-羟基喹啉-5-磺酸为掩蔽剂,分光光度法测定血清钙。对反应条件和方法性能进行系统研究。结果 该方法显色络合物最大吸收波长为580 nm,线性范围达5.O mmol/L,回收率为98.8%~99.1%,平均99.0%。批内变异系数(CV)和批间变异系数(CV)分别为0.95%和1.74%。与邻甲酚酞络合酮(OCPC)(X1)比较:Y=1.003X1—0.036,r=O.996;与偶氮胂Ⅲ(X2)比较:Y=1.001X2—0.030,r=O.997;与酶速率(X3)法比较:Y=0.999X3-O.045,r=O.998。在血清胆红素高达412μmol/L,血红蛋白7 g/L,镁2.50 mmol/L及Intralipid高达8 g/L时均对该法无显著干扰。结论该法具有简便、快速、试剂稳定和灵敏可靠的优点,适合血清钙的手工测定和自动分析。
Objective To establish a high sensitivity, good-selectivity and stable method for determination of calcium in serum by spectrophotometry with chlorophonazo I . Methods Chlorophonazo I was used as a coloring reagent and 8-hy-droxyquinoline-5-sulfonic acid as a reagent covering magnesium in the 2-amino-2-methyl-1, 3-propanediol-HCl buffer system to determine calcium by spectrophotometry. The systematic studies of reactions conditions and properties of the method were made. Results The maximum absorption of the complex compound was 580 nm. The linearity was 5. 0 mmol/L. The recoveries ranged from 99. 0% to 101. 5% and the average rate of recovery was 101. 1%. The within-run and between-run CVs were 2. 3% and 2. 9%. Correlation between results obtained by this method(Y)and OCPC (X1) was y=1. 003X, -0. 036,r = 0. 996;Arsenazo I (X2) was y=1. 001X2 -0. 030,r=0. 997 and the enzymatic method(X3) was y = 0. 999X3 - 0. 045,r = 0. 998. The assay showed no interference from as much as 412 μmol/L of bilibin, 7g/L of haemoglobin,2. 50 mmol/L of magnesium and 8 g/L of intralipid. Conclusion The method are simple, sensitive and stable, suitable for the routine manual or automatic analysis of calcium in serum.
出处
《现代检验医学杂志》
CAS
2002年第4期11-13,共3页
Journal of Modern Laboratory Medicine