摘要
目的建立仙灵骨葆颗粒的薄层鉴别及含量测定方法,为其质量控制提供依据。方法采用薄层色谱(TLC)法对方中补骨脂、丹参、续断进行定性鉴别;采用高效液相色谱法(HPLC)对方中淫羊藿的有效成分淫羊藿苷进行含量测定。结果薄层鉴别项斑点清晰,分离度好,阴性无干扰;含量测定项线性关系良好(r=0.9999),平均回收率为98.0%。结论本方法可以准确地进行定性、定量分析,可用于仙灵骨葆颗粒的质量控制。
Objective To establish the method of identify and the content determination for the quality control of Xianlingbubao Granules and provide a basis of quality control. Methods Psoraleae fructus,dipsaci radix,salviae mil tiorrhizae radix et rhizoma were identified by TLC,and the content of Epimedium glycoside was determined by HPLC. Result The specks in TLC are clear and the blank tests showed no interference. The linear relationship is good( r = 0. 9999) and the average recovery is 98. 0%. Conclusion The methed is accurate,reliable and available with promising reproducibility and can be used in controlling the quality of Xianlinggubao Granules.
出处
《湖北中医药大学学报》
2014年第2期43-46,共4页
Journal of Hubei University of Chinese Medicine
