慢性应激对大鼠胸腺指数和血清CORT、胸腺肽浓度的影响

Effects of Chronic Stress on Thymus Index and Concentration of Serum CORT and Thymosin in Rats

目的研究慢性应激对大鼠胸腺指数和血清糖皮质激素(CORT)和胸腺肽浓度的影响。方法大鼠随机分为对照组和应激组。应激组大鼠给予复合应激,即禁水12 h、禁食12 h、束缚6 h、昼夜颠倒、强迫游泳15 min、潮湿垫料24 h和45°浸水5 min7种应激刺激,每天随机一次,7 d为一个周期,每种刺激应用一次,共14 d。应激结束后取材,计算胸腺指数,酶联免疫吸附测定法检测血清CORT和胸腺肽的含量。结果应激组大鼠胸腺指数为(1.08±0.13)mg/g,与对照组[(1.45±0.11)mg/g]比较显著性减低,有统计学意义(P<0.05);应激组大鼠血清CORT浓度为(87.91±2.45)pg/m L,与对照组[(77.28±1.56)pg/m L]比较明显增高,有统计学意义;应激组大鼠血清胸腺肽的含量为(0.93±0.03)×103pg/m L,与对照组[(1.40±0.06)×103 pg/m L]比较显著性降低,有统计学意义(P<0.05)。结论慢性应激会降低大鼠胸腺指数,抑制胸腺肽的分泌功能。

Objective To To study the influence of acute stress on thymus index and concentration of serum CORT and thymosin in rats. Methods Rats were randomly divided into control group and stress group. Stress group rats were given compound stress,including absolute diet 12 h, water deprivation 12 h, being bounded 6 h, day and night inversion, forced swimming 15 min, damp bedding 24 h, 45° water immersion, which were randomly given once a day with 7 days for a cycle, and each stimulus was applied once for a total of 14 days. Then rats were killed and serum CORT and thymosin were extracted. We calculated the thymus index and determined the concentration of serum CORT and thymopeptide using enzyme-linked immunosorbent assay. Results The thymus index was(1.08±0.13)mg / g in the stress group, lower than(1.45±0.11)mg / g in the control group, and the difference was statistically significant, P<0.05. The CORT levels was(87.91±2.45) pg/ml in the stress group, higher than(77.28±1.56)pg/ml in the control group, and the difference was statistically significant, P<0.05; the concentration of serum thymosin was(0.93±0.03)×103 pg/ml in the stress group, lower than(1.40±0.06)×103pg/ml in the control group, and the difference was statistically significant, P<0.05. Conclusion Chronic stress can lower the thymus index and inhibit the secretion of the thymosin in rats.