肾素-血管紧张素系统阻断对肝脏部分切除小鼠HGF及TNF-α表达的影响

Renin Angiotensin System Blockade on Expression of Mouse HGF and TNF-alpha on Resection of Liver Sections

目的探讨应用卡托普利阻断肾素-血管紧张素系统后(RAS)对肝切除术小鼠肝功能及HGF及TNF-αm RNA表达的影响。方法于2014年6—12月随机选取40只雄性CBA小鼠,随机平均分为对照组与研究组,2组均予以70%肝切除术,研究组注射750 mg/(kg·d)卡托普利于腹腔,对照组每日注射等量生理盐水,对比2组术后3、6 d谷丙转氨酶(ALT)、谷草转氨酶(AST)等肝功能标志物以及HGF、TNF-αm RNA表达水平。结果研究组术后3 d平均ALT(59.2±2.4)u/l、AST(66.3±2.9)u/L,术后6 d平均ALT(15.7±2.9)u/l、AST(12.9±1.5)u/l,治疗3 d平均HGF表达(0.89±0.02)、TNF-α(0.71±0.04),6 d平均HGF表达(0.81±0.06)、TNF-α(0.53±0.03),小鼠肝脏部分切除术后血清ALT、AST指标,TNF-αm RNA、HGFm RNA表达水平均有变化,差异有统计学意义(P<0.05)。结论小鼠部分肝切除后RAS阻断利于调节肝功能、降低炎症发生率。

Objective To study the application of captopril block renin-angiotensin system(RAS) on liver function in mice liver resection and HGF and the influence of TNF alpha m RNA expression. Methods 40 male CBA mice from 2014 June to 2014 December, randomly divided into control group and research group, two groups are 70% hepatectomy, team daily dose of 750 mg/kg captopril in abdominal cavity, the amount of normal saline control group daily injection, compared two groups of postoperative 3 d,6 d cereal third transaminase(ALT), aspertate aminotransferase(AST), and other liver function markers, and HGF, TNF alpha m RNA expression level. Results After the resection of liver in mice serum ALT, AST, TNF alpha m RNA expression level, HGFm RNA have change. Conclusion Mice after partial liver resection RAS blocking conducive to regulating liver function, reduce the incidence of inflammation.