血管内皮生长因子及其受体在病变角膜的表达和临床意义的探讨

Expression of Vascular Endothelial Growth Factor and Its Receptors (flt-1) in Morbid Human Corneas and Investigation of Its Clinic Importance

目的:探讨血管内皮生长因子(vacular endothelial growth factor,VEGF)及其受体flt-1在各种病变角膜的表达和可能的作用。方法:收集正常周边角膜和角膜缘组织11例和各种病变的角膜标本32例。冰冻切片,用免疫组织化学方法检查标本内VEGF及其flt-1,分析其VEGF的表达与临床表现的关系。结果:VEGF在血管化和非血管化角膜标本均为阳性,在角膜上皮和病变基质表达较强;flt-1在大多数标本呈现阳性反应。在基质和内皮病变角膜VEGF表达高于正常角膜的表达(P<0.05),在浸润炎症细胞和增殖组织中的表达与角膜混浊程度、新生血管面积呈正相关(P<0.05)。结论:各种病变角膜均表达了VEGF及其受体,VEGF在角膜损伤的修复和新生血管化中可能起着重要作用。眼科学报 2002;18;203-207.

Purpose:To investigate the expression of vascular endothelial growth factor(VEGF) and its receptor(flt-1) in morbid corneas and the potential effects. Methods: Eleven cases of normal peripheral corneas and limbal tissues and 32 cases of miscellaneous morbid corneas were collected during corneal transplantation. The specimens were sectioned in a cryostat and stained with streptavidin-biotin-peroxidase method for VEGF and flt-1. The expressive levels of VEGF were compared with grades of patients' clinical manifestations. Results: Positive expression of VEGF was exclusively presented in all morbid corneal specimens and that of flt-1 was showed in most cases. Stronger staining was found in the epithelia and stoma of the morbid cornea. The expressive levels of VEGF were more important in the stroma and the endothelia of the morbid corneas than that in the normal peripheral corneas and were positively correlated with the degrees of corneal opacities and the area of corneal neovascularization in infiltrating inflammatory cells and proliferative tissues ( P < 0. 05). Conclusions: VEGF and flt-1 were expressed in miscellaneous morbid corneas and may play an important role in the repair of corneal injuries and the corneal neovascularization. Eye Science 2002; 18: 203 - 207.