摘要
目的:建立高效液相色谱法,同谱测定复方头孢羟氨苄胶囊中的头孢羟氨苄和甲氧苄啶含量。方法:用C_(18)柱,以pH3.5醋酸钠缓冲液—甲醇(77:23)为流动相,检测波长271nm。结果:头孢羟氨苄和甲氧苄啶进样量分别在3.2～4.8μg和0.64～0.96μg范围内,与相应主峰面积线性关系良好(γ分别为O.9996和O.9997),头孢羟氨苄和甲氧苄啶的平均回收率分别为99.9％和100.3％。结论:方法专属性强,简便快速,结果准确。
Objective: To establish a HPLC method for the assay of cefadroxil and trimethoprim inCompound Cefadroxil Capsules. Method: The chromatographic conditions were C_(18)column, pH3.5 sodiumacetate buffer-methanol (77:23) as mobile phase, and 271nm as detecting wavelength. Result: Both thestandard curves of cefadroxil and trimethoprim were rectilinear within the injecting quantity ranges of 3.2～4.8μg and 0.64～0.96μg respectively (γ were 0.9996 and 0.9997 respectively). The average recoveries of cefadroxil and trimethoprim were 99.9% and 100.3%. Conclusion: The method was specific,simple,andaccurate.
出处
《中国药品标准》
CAS
2002年第6期52-54,共3页
Drug Standards of China
