曲尼司特对单纯周围神经损伤后抑制瘢痕形成的实验研究

An experimental study on effects of tranilast on prophylaxis of a chronic constriction mononeuropathy

目的研究抗瘢痕增生药物曲尼司特在单纯慢性卡压性周围神经病变中的抗瘢痕形成作用。方法按照Bennett等报导的方法,将36只大鼠分为两组:实验组在整个实验过程中,每日经口给予曲尼司特200mg/kg,对照组每日给予药物溶剂10ml/kg。比较两组动物从7d到28d患肢热板试验、神经传导速度、组织病理学和电镜微细结构的改变。结果右下肢热逃避反射潜伏期和神经传导速度的改变,实验组较对照组明显变短,对照组坐骨神经在实验7d后表现为神经周围或神经束间炎性反应和轴索变性,14d大量的轴索发芽和再生的有髓纤维,28d神经束再生。实验组有效地预防了早期神经的炎性反应和轴索的变性。结论曲尼司特在大鼠单纯慢性卡压性神经病变模型中有对瘢痕形成抑制和神经功能的保护作用。

Objective To clarify the actions of tranilast,an anti allergic drug,on chronic constriction mononeuropathy through an experiment using an animal model according to the method of Bennett et al. Methods 36 rats were divided into 2 groups: one was treated with tranilast(200mg/kg·d-1,p.o every experimental day),the other was controlled with solvent only. Chronological changes of heat evoked withdrawal latencies on a hot plate,nerve coducting velocitics, and histopathological changes were compared between the two groups from one to four weeks after loose ligation in the sciatic nerve. Results The changes of the withdrawal latency and nerve conducting velocities in the tranilast treatment group were significantly longer than those in the control. The rats without tranilast treatment showed inflammation in and around the constricted nerve bundles,axonal degeneration,phagocytes invasion and interstitial edematous changes at 7 days,numerous axonal sprouts and remyelination at 14 days,and regeneration in the nerve bundles at 28 days. In contrast,the rats treated with tranilast showed less inflammation or nerve fiber degeneration,and better regeneration than those of the control. Conclusion The actions of tranilast appear to have beneficial effects on prophylaxis of a chronic constriction mononeuropathy and on preservation of the nerve functions in rats.