血管紧张素转换酶基因多态性与妊高征的相关性研究

Detection of insertion/detection polymorphism of angiotensim converting enzyme gene in preeclampsia.

目的 探讨血管紧张素转换酶 (ACE)基因第 1 6内含子插入片段 (Ⅰ )和缺失片段 (D)的多态性与妊娠高血压综合征 (PIH)的相关性。方法 采用聚合酶链反应 -限制性片段长度多态性检测 60例PIH孕妇 (PIH组 ) ,40例正常妊娠孕妇 (对照组 )的ACE基因插入与缺失多态性。结果 PIH组ACE基因DD型和Ⅱ型频率分别为 46 7%(2 8/ 60 )和 30 %(1 8/ 60 ) ,而对照组则分别为 2 5 %(1 0 / 4 0 )和 57 5 %(2 3/ 4 0 ) ,两组比较 ,差异有显著性 (P <0 0 5) ,PIH组Ⅰ等位基因频率为 0 4 ,D等位基因频率为 0 6 ,对照组分别为 0 7,0 3 ,两组比较 ,差异有极显著性 (P <0 0 1 )。结论 ACE基因DD型个体与PIH发病存在相关性。

Objective:to study a polymorphism and allece frequency of the angiotensin converting enzyme(ACE) gene in pregnancy induced hypertension (PIH).Methods:60 patients with preeclampsia and 40 normal pregnant women as controls were investigated. A pair of primers,for the intron 16 of ACE gene was designed. A sensitive and specific method for detecting the ACE polymorphism of the insertion/deletion(I/D) was established. Determined by polmerase chain reaction-restriction fragment 1ength polymorphism (PCR-RFLP). A 490bp(I) and 190bp(D) PCR products were indentified, corresponding to the PCR amplification of the allele with or without the insertion. Results: compared the percentage of the ACE DD II genotype (46 7%,30%) in the PIH patients with the percentage (25%,57 5%),they were significant higher in indiriduals with PIH(P<0 05). compared the frequency of allele gene (0 6) in the PIH patients with the frequency (0 3), they were significant higer in individuals with PIH (P<0 01). Conclusions:genotype DD of ACE is a risk gene,suggesting the ACE gene may contribute to the pathogenesis of PIH.