摘要
以黄早四 (抗 )×掖 10 7(感 )的 F2 分离群体 (184个单株 )为作图群体 ,构建了具有 6 5个 SSR标记位点的遗传连锁图谱 ,覆盖玉米基因组 1333.3c M,标记间平均距离 2 0 .5 c M。通过人工接种鉴定评价 184个 F3家系对 SCMV引起的玉米矮花叶病的抗性反应。采用复合区间作图法对抗病数量性状位点 (QTL)进行定位及遗传效应分析 ,结果共检测到 3个 QTL s,分别位于第 3、 6和 10染色体上 ,与标记 phi0 5 3、phi0 77和 phi0 6 2连锁。第 3染色体上的 QTL 效应最大 ,可解释表型方差的 17.8% ,基因作用方式为加性 ;第 6和第 10染色体上的 QTL 效应较小 ,分别解释表型方差的 6 .1%和 7.0 % ,基因作用方式均为部分显性。采用多区间作图法未检测到 QTL s间显著的上位性互作。 3个 QTL s共解释表型方差的 30 .2 %。
The genetic linkage maps was constructed with sixty five SSR markers based on a maize population consisting of 184 F 2 individuals from cross HangZao4 (resistant)×Ye107 (susceptible), covering 1333.3 cM on total ten chromosomes with an average interval length of 20.5 cM. The population of 184 F 3 lines was evaluated for Sugarcane Mosaic Virus (SCMV) resistance under artificial inoculation. With the method of composite interval mapping, three QTL conferring resistance to SCMV were identified on chromosome 3, 6, and 10, Linked with markers phi053, phi077 and phi062, and explained 17.8%, 6.1%, and 7.0% of the phenotypic variance, respectively. The gene action was additive for the QTL on chromosome 3, while partially dominant for the QTL on chromosome 6 and 10. No significant epistatic interactions were found between QTLs by the method of multiple interval mapping, and three QTLs together explained 30.2% of the phenotypic variance.
出处
《作物学报》
CAS
CSCD
北大核心
2003年第1期69-74,共6页
Acta Agronomica Sinica
基金
亚洲开发银行玉米生物技术协作网 (AMBIONET)项目资助
