光动力治疗裸鼠胰腺癌及其机理的实验研究

Experimental Study of the Therapeutic Effect and Mechanism of Photodynamic therapy to Pancreatic Cancer by Intratumoral Injecting Photosensitizer

目的 探讨在裸鼠胰腺癌瘤内注射光敏剂血卟啉衍生物 (HpD)、竹红菌甲素 (HA)及 2 丁胺 2 去甲氧基竹红菌甲素 (2 BA 2 DMHA)后 ,光动力治疗 (PDT)的效果及其作用机理。方法 将人胰腺癌细胞株SW1 990接种到裸鼠皮下 ,建立胰腺癌动物模型 ,而后将光敏剂HpD、HA及 2 BA 2 DMHA注射入肿瘤 ,再用激光照射肿瘤局部 ,观察PDT治疗胰腺癌的效果。另以第Ⅷ因子为标记 ,采用免疫组化LSAB法研究肿瘤血管损伤的情况。结果 PDT对胰腺肿瘤有明显杀伤作用 ,能使胰腺肿瘤生长速度明显减慢。免疫组化染色显示 ,PDT可引起血管内皮细胞损伤和血管结构破坏。结论 PDT对胰腺癌有治疗作用 。

Objective To study the therapeutic effect and mechanism of photodynamic therapy (PDT) to the nude mice model of pancreatic cancer by intratumoral injecting photosensitizers hematoporphyrin derivatives (HpD), hypocrellin A (HA) and 2 butylamino 2 demethoxy hypocrellin A (2 BA 2 DMHA).Methods The animal model of human pancreatic cancer was developed by injecting human pancreatic cancer cells SW1990 into the back of the nude mice. After photosensitizers HpD, HA and 2 BA 2 DMHA was given by the intratumoral injection, the 632.8 nm He Ne laser was used to irradiate the tumor. The curative effect was recorded and factor Ⅷ was used in the immunohistochemical staining to study the vessel change. Results PDT can destroy the pancreatic neoplasm, the tumor growth rate was significantly reduced after PDT. The immunohistochemical staining showed PDT could make injury to vessel endothelial cell. Conclusion PDT can induce injuries of pancreatic cancer; vascular injury is an important way to exert function.