慈菇对镉致肝脏脂质过氧化及c-fos mRNA表达的影响

Effect of Sagittara Sagittifolia on changes of liver lipid peroxidation and expression of c-fos mRNA induced by cadmium

目的 探讨慈菇对镉致肝脏脂质过氧化及c -fosmRNA表达的影响。方法 3 6只雄性SD大鼠随机分为 6组 (n =6) ,分别用 2 5 %、5 0 %、75 %的慈菇 (2ml/ 10 0g体重 ) ,维生素E(5 0mg/ 10 0g体重 )及等体积蒸馏水灌胃 ,连续 10d ,末次灌胃 2 4h后 ,腹腔注射CdCl2 (2 0 μmol/kg体重 ) ,染毒 2 4h后 ,比较各组肝脏丙二醛 (MDA)、谷胱甘肽 (GSH)含量及谷胱甘肽过氧化物酶 (GSH -PX)活性 ;并利用反转录聚合酶链式反应 (RT -PCR)对肝脏c -fosmRNA进行半定量测定。结果 VE和中、高剂量慈菇都降低了镉所升高的MDA含量 (P <0 0 5 ) ,中、高剂量的慈菇还提高了氯化镉所降低的肝脏GSH含量 (P <0 0 5 ) ;而VE和各剂量慈菇都不能纠正氯化镉所升高的c -fosmRNA水平 (P >0 0 5 )。结论 镉对肝脏c

Objective To study the effect of liver lipid peroxidation on expression of c-fos mRNA induced by cadmium.Methods The male Sprague-Dawley rats were randomly assigned into six groups,each of six.The rats were pretreated intragastrically with 25%,50%,75% Sagittaria Sagittifolia extract(2?ml/100?g·bw),vitamin E(50?mg/100?g·bw) or distilled water once daily for ten days.Twenty-four hrs after the last dosing,animals were administered with CdCl 2 (20?μmol/kg) intraperitoneally.Control animals were given distilled water only.Twenty-four hrs after the intoxication,the liver MDA,GSH content and GSH-PX activity were measured in each group above;liver c-fos mRNA was also semi-quantified by reverse-transcription polymerase chain reaction(RT-PCR).Results Pretreatment of rats with 5% and 75% Sagittaria Sagittifolia extract and vitamin E could decrease liver MDA content( P <0 05),the former also increased liver GSH content( P <0 05);However,both pretreatment of rats with Sagittaria Sagittifolia and vitamin E could not decrease liver c-fos mRNA levels induced by cadmium( P >0 05).Conclusion Lipid peroxidation seemed not or partially to involve in inducing expression of c-fos mRNA in the process of acute cadmium exposure.