甲基莲心碱增强阿霉素对骨肉瘤的抑制作用及机制初探

Enhancement of cytotoxicity of Adramycin by neferine in Saos-2 cells and its mechanism

目的 研究甲基莲心碱 (Nifeine ,Nef)与化疗药物合用时 ,对骨肉瘤的化疗增敏作用及其机制。方法 用MTT法测定药物的细胞生长抑制率 ,PI染色流式细胞术检测细胞周期和细胞凋亡。间接免疫荧光流式细胞术和S P免疫组化检测蛋白表达。结果 5 ,10 μmol·L-1Nef能降低ADM对Saos 2的IC50 。 10 μmol·L-1Nef增加了ADM诱导的Saos 2凋亡 ,增强了细胞的Rb蛋白表达 ,使G1期细胞增多。结论 Nef能增加ADM对Saos 2的化疗敏感性 ,其机制可能与增强Saos

AIM To study the synergistic anticancer effccts of neferine combined with anticancer agents on Saos 2 cell lines and its mechanism. METHODS\ Cytotoxicity assay was tested by MTT method.Cell cycle and apoptosis was detected by PI staining flow cytometty. Expression of Rb protein was examined by indirect immune fluorescent symbol flow cytometty and S P immunohistochemical staining. RESULTS\ 5,10 μmol·L -1 Nef augmented apoptosis of Saos 2 cells induced by ADM,enhanced the expression of Rb protein in Saos 2 cells and raised the G 1 phase ratio of Saos 2 cells. CONCLUSION Nef can promote chemotherapy sensitivity to ADM in Saos 2 cells. Its mechanism is associatd with the up modulation of Rb protein expression in the Saos 2 cells arrest cells in G 1 phase