斑秃患者血浆和皮损中降钙素基因相关肽、血管活性肠肽的研究

Calcitonin Gene-related peptides and Vasoactive Intestinal Peptide in Plasma and Lesion of Patients with Alopecia Areata

目的探讨降钙素基因相关肽(CGRP)和血管活性肠肽(VIP)在斑秃发病中的作用。方法利用放射免疫分析法检测30例斑秃患者和20例正常人血浆中的CGRP和VIP水平,利用免疫组化方法检测21例斑秃患者皮损和16例正常人头皮中的CGRP和VIP表达情况。结果①斑秃活动期患者血浆中的CGRP水平为(142.63±67.95)pg/mL,低于稳定期(197.33±67.15)pg/mL和正常人(188.40±72.95)pg/mL,差异均有显著性。②斑秃活动期血浆中的VIP水平为(105.94±55.42)pg/mL,低于斑秃稳定期(156.86±47.37)pg/mL和正常人(176.44±84.70)pg/mL,差异均有显著性。③CGRP和VIP在斑秃皮损及其周围的表达明显低于正常人,差异有显著性。结论CGRP和VIP在斑秃发病中起一定的作用。

Objective To study the role of calcitonin gene-related peptide(CGRP)and vasoactive intestinal peptide(VIP)in the pathogenesis of alopecia areata(AA).Methods Radioimmunoassay(RIA)was used to measure the levels of CGRP and VIP in plasma from30patients with AA and20normal controls.Immunohistochemistry was employed to detect the expression of CGRP and VIP in lesions of21patients with AA and16normal scalps.Results①The plasma levels of CGRP in progressing stage of AA(142.63±67.95pg/mL)were significantly lower than those in stable stage of AA(197.33±67.15pg/mL)and in normal controls(188.40±72.95pg/mL).②The plasma levels of VIP in progressing stage of AA(105.94±55.42pg/mL)were significantly lower than those in stable stage of AA(156.86±47.37pg/mL)and in normal controls(176.44±84.70pg/mL).③The expression of CGRP and VIP was significanly decreased in lesions of AA than that in normal scalps.Conclusion These findings indicate that CGRP and VIP may play a role in the pathogenesis of alopecia areata.