196味中药乙醇提取物对酪氨酸酶的抑制作用

Inhibitory Effects of Ethanolic Extracts From196Kind of Chinese Herbs on Tyrosinase

目的评估196味中药乙醇提取物对酪氨酸酶的抑制作用。方法用多巴色素法测定对酪氨酸酶的抑制作用,底物为L-多巴,在475nm处测定透过率。结果通过筛选196味中药发现光果甘草、五倍子、蝉衣、牡丹皮、苦参、珍珠绣线菊、苍耳子、桑树根皮(鲜)、大黄(生)在高浓度(500μg/mL)时对酪氨酸酶的抑制作用超过浓度为1mmol/L的熊果苷的抑制作用(P<0.01),其中以光果甘草的抑制作用最强(高浓度时抑制率达98.3%);六月雪、泥湖菜、地骨皮、炒川断、天麻、连翘、茶条槭、甘草、败酱草、黄杨(干叶)、双花等11味在高浓度(500μg/mL)时与浓度为1mmol/L的熊果苷的抑制作用相当(P>0.05);茵陈、威灵仙、白芨、紫槿、大蓟、小构树、钩藤、玉竹、熟大黄、莪术、苍术、姜黄、黄芩、虎杖、豚草、狭叶山胡椒、决明子乙醇提取物对酪氨酸酶同时有抑制作用和激活作用。结论光果甘草、五倍子、蝉衣、牡丹皮、苦参、珍珠绣线菊、苍耳子、桑树根皮(鲜)、大黄(生)、六月雪、泥湖菜、地骨皮、炒川断、天麻、连翘、茶条槭(包括阴干组和新鲜组2种)、甘草、败酱草、黄杨(干叶)、双花等20味中药具有治疗因酪氨酸酶活性异常所致的色素增加性皮肤病的应用前景。

Objective To evaluate the inhibitory action of ethanolic extracts from196kinds of Chi-nese herbs on tyrosinase.Methods Tyrosinase inhibitory activity was determined by the dopachrome method using L-DOPA as the substrate and the amount of dopachrome in the reaction mixture was measured by spec-trophotometer.Results Nine Chinese crude extracts[Glycyrrhiza glabra L.,Melaphis chinensis(Bell.)Baker,Cryptotympana atrata Fabricius,Paeonia suffruticosa Andr.,Sophora flavescens Ait.,Spirea thunbegii Sieb.et Bl.,Xanthium sibiricum Patr.,Morus alba L.,Rheum palmatum L.]showed potent inhibitory action on tyrosi-nase compared with positive control arbutin(1mmol/L,P<0.01),and11Chinese extracts[Artemisia anomala S.Moore,Hemistepta lyrata Bunge,Lycum chinensis Mill.,Dipsacus asper Wall.,Gastrodia elata Bl.,Forsythia suspensa(Thunb.)Vahl.,Acer ginnala Maxim.,Acer ginnala Maxim.,Glycyrrhiza uralensis Fisch.,Patrinia sabiosaefolia Fisch.,Buxus sinica(Rehd.et Wils.)Ching,Lonicera japonica Thunb.]showed no difference com-pared with positive control arbutin(1mmol/L,P>0.05).Conclusion The results suggest that20kinds of crude extracts from Chinese herbs[Glycyrrhiza glabra L.,Melaphis chinensis(Bell.)Baker,Cryptotympana a-trata Fabricius,Paeonia suffruticosa Andr.,Sophora flavescens Ait.,Spirea thunbegii Sieb.et Bl.,Xanthium sibiricum Patr.,Morus alba L.,Rheum palmatum L.,Artemisia anomala S.Moore,Hemistepta lyrata Bunge,Lycium chinensis Mill.,Dipsacus asper wall.,Gastrodia elata Bl.,Forsythia suspensa(Thunb.)Vahl.,Acer gin-nala Maxim.,Glycyrrhiza uralensis Fisch,Patrinia sabiosaefolia Fisch.,Buxus sinica(Rehd.et Wils.)Ching,Lonicera japonica Thunb.,]inhibit tyrosinase and may be used as depigmentaty agents for pigmentary skin dis-eases caused by abnormal tyrosinase activity.