影响黄体酮促两种沼虾卵巢发育作用的一些因素

FACTORS AFFECTING PROGESTERONE EFFECT ON OVARY DEVELOPMENT OF TWO SPECIES OF MACROBRACHIUM PRAWN

在11月给不切眼柄的日本沼虾注射黄体酮和滴加黄体酮,对其卵巢发育无明显的作用。切眼柄组比不切眼柄组有更多的虾达到较成熟的卵巢发育期,而切眼柄又注射或滴加黄体酮的组又比仅切眼柄的组有更多的虾达到较成熟的卵巢发育期。结果表明,眼柄内的激素可抑制黄体酮对卵巢发育的作用。10-9M和10-12M黄体酮可使体外培养的罗氏沼虾Ⅱ期和Ⅲ期卵巢的卵母细胞直径显著增大或非常显著地增大,而这2种浓度的黄体酮对Ⅰ期和Ⅳ期的卵巢的卵母细胞的直径都无增大作用。10-9M和10-12M的黄体酮可使体外培养的罗氏沼虾Ⅱ期和Ⅲ期卵巢的总蛋白合成较对照组非常显著或显著地增加,而这2种浓度的黄体酮对Ⅰ期和Ⅳ期的卵巢的总蛋白合成都无增加作用。结果表明黄体酮对卵母细胞的作用是有发育期的选择性的。

By injecting into intact Macrobrachium nipponesis or dropping on its body face progesterone in November could not stimulate ovary development of the prawn. In groups of eyestalk ablation more prawns developed ovaries in more advanced stages than in group of intact prawns In groups where prawns were treated with eyestalk ablation and injecting or dropping of progesterone more ovaries in more developing stages were observed than in group in which the prawns were only treated with progesterone. The results show that hormone in the eyestalk of prawns can inhibit the progesterone effect on the ovary development of M. nipponesis . Progesterone at concentrations of 10-9M and 10-12M only significantly increased the oocyte diameter of ovaries in stages Ⅱand Ⅲ of M. rosenbergii and promoted the syntheses of total protein of the ovaries in the same development stages in vitro. The results indicate that the progesterone effect on the ovary development is stage-specific.