肿瘤标志物CDw75在胃癌组织中的表达及其临床意义

Expression of Tumor Marker CDw75and Its Clinical Impact in Gastric Carcinoma

背景和目的:最近的研究表明,涎酸化糖轭合物的肿瘤相关表达与恶性肿瘤细胞的侵袭行为和转移潜能密切相关。CDw75(clusterofdifferentiationantigen75)是由β-半乳糖基α-2,6-涎酸转移酶(β-galactosylα2,6-sialyltransferase,Sia-T1)产生的一类涎酸化糖轭合物,是新近发现的一种肿瘤标志物。本研究观察CDw75在胃癌组织及正常胃粘膜中的表达,探讨其与胃癌浸润、转移和临床分期的关系。方法:用免疫组化法检测CDw75抗原在54例胃癌组织及其相应正常胃粘膜组织中的表达,经计算机图像分析,计算阳性细胞百分率。结果:正常胃粘膜中未见CDw75抗原表达,而在胃癌组织中有26例(48.1%)呈阳性表达,两者差异有统计学意义(P<0.05),且CDw75抗原表达与肿瘤浸润深度、临床TNM分期及淋巴结转移相关性有统计学意义(P<0.05或<0.01)。结论:胃癌组织中CDw75的表达可作为胃癌生物学行为的标志。

BACKGROUND &OBJECTIVE:Tumor-associated expression of sialylated g lycoconjug ates was found to be closely associated with ag g r essive behavior and increased metastatic p otential of malig nant cells.The CDw75(cluster of differentiation antig en75)epitope is a sialylated carbohydrat e determinant g enerated by β-g alactosyl α2,6-sialyltransferase.This study was desig ned to investig ate the expression of CDw75in g astric carcinoma and normal g astric mucosa,and the relationship between its exp ression and the infiltration,metastasis ,and clin ical stag e of g astric cancer.METHODS :The expression of CDw75antig en in 54cas es of g astric carcinoma and normal g astric mucosa was detected using im munohistochemical method.The percentag e of CDw75positive cells w as counted throug h the computer anal ysis of picture.RESULTS :There was no expression of CDw75antig en in normal g astric mucosa,while positive stai ning was detected in 26(48.1%)of the 54g astric cancer (P<0.05).The expression of CDw75antig en was sig nificantly associated with invasive depth,TNM stag e,and lymph node metastasis(P<0.05or P<0.01).CONCLUSION:The expression of CDw75in tumor tiss ue may be a new marker of the biolog ical b ehavior of g astric carcinoma.