反相高效液相色谱法测定保健食品中的枸橼酸西地那非

Detection of sildenafil citrate from health food by using reversed-phase high performance chromatography

目的 建立一种测定保健食品中枸橼酸西地那非的可行方法。方法 采用反相高效液相色谱法 ,操作条件 :ZORBAXODS 4.6mmi.d× 2 5 0mm为色谱柱 ,柱温为 40℃ ,0 .0 5ml/L磷酸三乙胺 (pH =3.3) :甲醇 :乙腈 =5 0 :30 :2 0(V :V :V)为流动相 ,流速为 1.0 0ml/L ,检测波长为 2 90nm。结果 其工作曲线的线性范围 :0 .0 8～ 2 .0 0 μg ,相关系数 :r =0 .9999,回收率 :94.0 %～ 98.6% ,精密度 (n =6) :1.85 %～ 1.89%。结论 所建立的方法准确、可靠 。

Objective To set up a practical method for determination of sildenafil citrate in healthy foods.Methods Reversed-phase high performance liquid chromatography was used. The operating conditions were ZARBAX ODS column(4.6mm i.d×250mm)at 40℃,0.05mol/L phosphoric acid and triethylamine(pH=3.3):methanol:acetonitrile=50:30:20(V:V:V)as mobile phase with flow rate 1.00ml/min and UV detection at 290nm.Results The linear range of the calibration curve of sildenafil citrate were from0.08～2.00μg with a correlation coefficient(r)of 0.9999,The recoveries were 94.0%～98.6% and the relative standard deviations(n=6)were 2.48%～2.50%.Conclusion The method was accurate,realiable and can be used in determinating sildenfil citrate in he-althy foods.