β-榄香烯诱导结肠癌Lovo细胞凋亡的作用

Effects of Apoptosis Induced by β-Elemene in Colon Cancer Lovo Cell Line

目的 研究 β 榄香烯诱导结肠癌细胞的凋亡作用 ,探讨其治疗结肠癌的作用机制。 方法 选择 β 榄香烯诱导结肠癌细胞凋亡 ,采用光镜、琼脂糖凝胶电泳和流式细胞仪检测凋亡及细胞周期。Fura 2荧光复合技术测结肠癌细胞内 [Ca2 +]i浓度。结果 β 榄香烯对结肠癌细胞增殖具有很强的抑制作用。β 榄香烯能够诱导结肠癌凋亡。在结肠癌细胞凋亡过程中 ,胞内 [Ca2 +]i明显升高 ,以凋亡早期更为明显。 1h时为 ( 2 5 6.18± 8.85 )nmol/L ,明显高于对照组 ( 12 7.81± 5 .18)nmol/L(P <0 .0 1)。结论 β 榄香烯对结肠癌细胞生长具有很强的抑制作用 ,与诱导凋亡有关。Ca2 +在 β

Purpose. To explore the mechanism of β-elemene on colon carcinoma Lovo cell line. Methods. Morphologic changes, supression rate, apoptosis of colon carcinoma Lovo cell line were studied by light microscopy, MTT assay, light and agarose gel electrophoresis and flow cytometry. Apoptosis induced by β-elemene in colon carcinoma Lovo cell line was investigated by [Ca 2+]i was determined by Fura-2 fluorescin load method. Results. β-elemene had a remarkable supression effect on colon carcinoma Lovo cell line. Anticancer effect of β-elemene would be correlation with apoptosis. [Ca2+]i was significantly increased, especially at the initial stage of apoptosis. It is (256.18 ± 8.85)nmol/L at 1 h, which was significantly control (127.81 ± 5.18)nmol/L (P 2+]i play an important role in the process of β-elemene induced apoptosis of colon carcinoma cells.