中国畲族人群DYS287位点多态性的研究

An Investigation of Y Chromosome Alu Insertion Polymorphism in Chinese She Nationality

目的 :分析Y染色体特异的Alu插入位点DYS2 87的遗传多态性 ,并对江西、浙江和福建三省不同地区的畲族人群Alu插入频率进行比较。方法 :以中国畲族群体的 117名男性个体为研究对象 ,采用PCR -SSP法扩增后琼脂糖凝胶电泳分离的方法检测结果。结果 :我国畲族人群中Alu插入的基因频率为 17.1% ;江西、浙江和福建三省不同地区的畲族人群中Alu插入频率无显著差异。结论 :DYS2 87位点作为一种稳定、可靠的遗传标记可以为研究民族间的遗传关系及其起源提供可靠的证据。

Objective:To investigate the polymorphism of DYS287 in Chinese She Nationality.Method:YAP element was detected by PCR-SSP amplification and agarose gel electrophoresis.Result:20 individuals out of 117,YAP elementis present and the PCR product is 455bp,the YAP element's frequency in Chinese She Nationality is 17.1% ,the Alu insertion frequency is no obvious difference of She Nationality from JiangXi,ZheJiang and FuJian province.Conclusion:As an important and stable genetic marker,DYS 287 can provide reliable evidence in national genetic relation and origin study.