卡氏肺孢子虫性肺炎的动物模型建立及病原学观察

Establishment and model of Pneumocystis carinii pneumonia in animals and observation of its pathogen

[目的 ]为开展卡氏肺孢子虫的病原学形态观察、生物学特性以及药物治疗的研究 ,建立卡氏肺孢子虫肺炎的实验大鼠模型 .[方法 ]给SD实验大鼠皮下注射醋酸可的松后 ,制作肺组织印片 ,采用姬氏染色、肺印片六亚甲基四胺银染色 ,用油镜观察 .[结果 ]第 4周包囊检出率为 6 7% (4 / 6 ) ,第 5周为89% (8/ 9) ,第 6周为 10 0 % (9/ 9) ,总检出率为 75 % (2 1/ 2 8) ,且随用药时间的延长 ,其感染程度也逐渐加重 .对照组均未检出包囊 .[结论 ]建立了卡氏肺孢子虫性肺炎的实验动物模型 。

OBJECTIVETo carry out the observation of pathogen, biologic characteristic of Pneumocystis carinii and study of medication, experimental model of Pneumocystis carinii pneumonia (PCP) in rats was established.METHODSSD rats were immunosuppressed with injection of cortisone acetate, and the klatsch-preparations of lung were observed by high power with staining of giemsa and gomori's methenamine silver nitrate stain.RESULTSThe detectable rate of Pneumocystis carinii cysts was 67%(4/6) in fourth weeks, 89%(8/9) in fifth weeks, 100%(9/9) in sixth weeks, respectively, and the total was detectable rate was 75%(21/28).CONCLUSIONThe model of PCP in rats had been successfully established.