葛根总黄酮治疗去势大鼠萎缩性咽炎的实验研究

The effect of the purariae-isoflavones on atrophic pharyngitis in ovariectomized rats

目的实验研究葛根总黄酮对去势大鼠萎缩性咽炎的治疗效果并初步探讨其机制。方法将60只成年雌性大鼠随机分成4组,每组各15只。①正常对照组;②去势组;③去势+胃灌注维尼安治疗组;④去势+胃灌注葛根总黄酮治疗组。抽血测定血清雌二醇(E2)和促黄体生成素(FSH)水平;对各组动物咽粘膜进行光镜观察。结果去势后,E2显著下降,FSH升高(P<0.05);经维尼安治疗,E2、FSH水平更趋于下降(P<0.05);经葛根总黄酮治疗后,E2恢复正常(P>0.05),FSH升高(P<0.05)。去势后30天咽粘膜呈萎缩性改变;经维尼安治疗30天,葛根总黄酮治疗60天后,咽粘膜恢复正常。结论葛根总黄酮可恢复去势大鼠的雌激素水平,对去势大鼠萎缩性咽炎有较好的治疗作用。

Objective To study the effect of purariae-isoflavone on atrophic pharyngitis in ovariectomized rats. Methods 60 rats were divided into four groups randomly, with 15 rats in each group, ①normal control group (CG); ②ovariectomized group without any treatmen(OG); ③ovariectomized group supplemented with weinian by gastrogavage (WG); ④ovariectomized group supplemented with purariae-isoflavone by gastrogavage (PG) group. Estrodiol (E2) and FSH of every rat were measured before and after treatment. Morphological changes of pharynx a-mong tested aminals were observed by light-microscopy. Results After being ovariectomized, E2 was lowered and FSH was increased compared with those of CG (P<0. 05). Both E2 and FSH of WG were lower significantly than those of CG and OG (P<0. 05). E2 was recoverd (P>0. 05), FSH was higher than those of CG and OG (P<0. 05) in PG. Atrophic changes of pharyngeal mu-cosa occurred 30 days after being ovariectomized. The lesion was recovered after being treated with Weinian for 30 days or with purariae-isoflavone for 60 days. Conclusion Purarize-isoflavone holds better therapeatic effects on experimental atrophic pharyngitis and can bring E2 level in serum to normal state for tested animals.