神经生长因子对吲哚美辛诱导人胚胎视网膜色素上皮细胞凋亡的保护作用

Nerve growth factor prevents apoptosis induced by indomethacin in cultured human fetal retinal pigment epithelial cells

目的 探讨神经生长因子 (nerve growth factor,NGF)对体外培养的人胚胎视网膜色素上皮(human fetal retinal pigment epithelium,HFRPE)细胞凋亡的保护作用。 方法 用传代培养的 HFRPE细胞 ,建立吲哚美辛 (indomethacin,IN)诱导的细胞凋亡模型 ,并用吖啶橙 (acridine orange,AO)荧光染色计数和透射电镜 (transmission electron microsocpy,TEM)观察 NGF对 HFRPE细胞凋亡的保护作用。 结果 用 AO荧光染色及 TEM均观察到经 2 0 0～ 6 0 0 μmol/ L IN处理 2 4 h后的 HFRPE细胞出现典型的凋亡形态学改变。 2 0 0 μmol/ L IN+5 0 0 μg/ L NGF组与对照组 2 0 0 μg/ (mol· L) IN相比凋亡细胞差异有显著性的意义 (q=3.92 0 4 ,P=0 .0 32 0 ) ;4 0 0μmol/ L IN+5 0 0μg/ L NGF组与对照组 4 0 0μmol/ L IN相比凋亡细胞差异有非常显著性的意义 (q=9.70 915 ,P=0 .0 0 0 1)。 结论 NGF能拮抗 IN诱导的 HFRPE细胞凋亡。

Objective To investigate the protective effect of nerve growth factor (NGF) on apoptosis of cultured human fetal retinal pigment epithelium (HFRPE) cells induced by indomethacin (IN) in vitro. Methods Subcultured HFRPE cells were treated with different concentrations of IN to establish apoptotic model. The protective effect of NGF on apoptosis of cultured HFRPE cells were assessed using an acridine orange (AO) staining method and transmission electron microscopy (TEM). Results HFRPE cells exposed by 200 600 μmol/L IN for 24 hours elicited typical apoptosis morphological changes, including condensed chromation, nuclear fragmentation and reduction of nuclear size and cell volume. There was a statistically difference in HFRPE cells with apoptosis between 200 μmol/L IN+500 μg/L NGF and 200 μmol/L IN groups ( q=3 9204,P=0.0320) ; there was a significant difference in HFRPE cells with apoptosis in 400 μmol/L IN+500 μg/L NGF and 400 μmol/L IN as well ( q=9 7915,P=0 0001). Conclusion NGF has an protective effect on IN induced HFRPE cells apoptosis.