PD98059对慢性髓细胞白血病Ras-MAPK信号转导的作用

Effect of PD98059 on Ras-MAPK signal transduction pathway of chronic my-elogenous leukemia

目的:探讨PD98059对慢性髓细胞白血病信号转导的作用及其作用机制。方法:将PD98059加入慢性髓细胞白血病细胞系K562细胞中,通过细胞活力、DNA合成、集落形成和MAPK活性的变化,观察PD98059对慢性髓细胞白血病信号转导的作用。结果:PD98059可明显抑制K562细胞活力、DNA合成、集落形成和MAPK活性(与对照组比较,P<0.05),其抑制作用具有浓度依赖性。结论:PD98059可通过阻断Ras-MAPK信号通路而发挥其抑制作用。Ras-MAPK通路极有可能成为CML治疗的新靶点。

AIM: TO study the effect and mechanism of PD98059 on Ras-MAPK signal transduction pathway of chronic myeloge-nous leukemia. METHODS: K562 cell line was treated by PD98059, cell viability, DNA synthesis, colony formation and MAPK activity of the treated cells were analyzed. RESULTS: The cell viability, DNA synthesis, colony formation and MARK activity were significantly inhibited by PD98059 (P<0.05), and the inhibitory effect was dose dependent. CONCLUSION: The inhibitory effect of PD98059 was achieved by blocking Ras-MAPK signal transduction pathway which can become a new target in the treatment of CML.