Th1、Th2样细胞因子对CD158^+细胞比率的调节作用

Modulation of the rate of CD158a^+/b^+ Cells by Th1- and Th2-like cytokines

目的:观察Th1、Th2样细胞因子对外周血CD158+细胞比率的调节作用,为寻找干细胞移植免疫耐受的方法提供论理依据。方法:将Th1样细胞因子(IL-2、IFN-γ)和Th2样细胞因子(IL-4、IL-6),单独或联合与人外周血单个核细胞(PBMC)培养72h,用流式细胞法分析总CD158a+、CD158b+细胞及CD3+、CD4+、CD8+和CD16+ CD56+细胞亚群中CD158a+、CD158b+细胞的比率。结果:①细胞因子对CD3+、CD4+、CD8+细胞及CD16+ CD56+细胞的影响:IL-2和IFN-γ均可增加上述细胞的百分率(P<0.05),但IL-2的作用大于IFN-γ(P<0.05)。IL-2+IFN-γ联合处理的效应高于IFN-γ单独处理(P<0.05)。IL-4+IL-6可降低上述细胞的百分率(P<0.05)。IL-2+IL-4对上述细胞百分率的影响,高于IL-4(P<0.05)但低于IL-2(P<0.05)。②细胞因子对CD158a+/b+细胞的影响:IL-2可增加总的CD158a+/b+细胞及CD3+、CD4+、CD8+和CD16+ CD56+细胞中的CD158a+/b+细胞的百分比率(P<0.05);IL-2+IFN-γ可增加CD158a+/b+细胞的百分率(P<0.05),但与IL-2单独处理无明显区别,IL-4+IL-6可降低CD158a+/b+细胞的百分率(P<0.05)。IL-2+IL-4可增加总的CD158a+/b+细胞及各亚群中CD158a+/b+细胞的百分率(P<0.05),但低于IL-2单独处理(P<0.05)。结论:IL-2有促进CD158基因表达或使CD158a+、CD158b+?

AIM: To explore modulation of CD158+ cells in human peripheral blood by Th1- and Th2-like cytokines and provide basic data for inducing immune tolerance and preventing graft-verse-host disease (GVHD) in stem cells transplantation. METHODS: Periphral blood mononuclear from health adults were cultured with Th1- like cytokines IL-2, IFN-γ and Th2- like cytokines IL-4, IL-6 for 72 hours, rates of CD3+, CD4+, CD8+ cells, CD16+ CD56+ cells and CD158a+/b+ cells were analyzed by FACS. RESULTS: ①The effects of cytokines on CD3+, CD4+, CD8 + and CD16+ CD56+ cells: the rates of above cells were greatly increased after being treated with IL-2 or IFN-γ( P<0.05), but efficaly of IL-2 was higher than that of IFN-γ(P <0.05). The rates of above cells in IL-2 + IFN-γ treated cells were higher than that in IL-2 or IFN-γ treated alone. The rates of above cells were greatly decreased after being treated with IL-4 + IL-6 ( P < 0.05), but efficacy of combination of IL-2 + IL-4 was higher than that of IL-4 alone, lower than that of IL-2 alone (P<0.05). ②The effects of cytokines on CDl58aVb* cells-, the rates of CD158aVb+ cells in total mononuclear and in CD3+, CD4+, CD8+ and CD16+ CD56+ cells were significantly raised after being treated with IL-2 (P<0.01), but had no significance changes after being treated with IFN-γ. The rates of CD158a+/b+ cells were decreased after being treated with IL-4 + IL-6, whereas increased after being treated with IL-2 + IFN-γ ( P < 0. 05), but efficacy of being treated with IL-2 + IL-4 was lower than that with IL-2 ( P < 0. 05). CONCLUSION: IL-2 plays an important role in the regulation of CD158a/b expression or proliferation of CD158a+/b+ cells. It may involve in controlling NK cells and T cells activity via expression of regulating these molecules or stimulating proliferating of CD158a+/b+ cells. IL-4 and IL-6 have a slight ability to decrease the rates of CD158a+/b+ cells and IL-4 can partially reverse the effect of IL-2 on CD158a+/b+ cells.