噬菌体展示ICAM-1模拟肽的制备及活性鉴定

Preparation and identification of phage-displayed ICAM-1-mimic peptide

目的:获得具有生物学活性的细胞间黏附分子-1(ICAM-1)模拟肽。方法:以噬菌体扩增及PEG沉淀法,大量制备展示十二肽P1、P2的噬菌体。采用ELISA、竞争抑制试验及免疫组化染色法,分别鉴定P1、P2模拟ICAM-1分子的结合特性及生物学活性。结果:噬菌体展示肽P1、P2能与抗ICAM-1单克隆抗体(mAb)15.2特异性结合。该结合作用可被ICAM-1分子竞争性抑制,P1、P2能模拟ICAM-1分子与LFA-1结合的功能。结论:噬菌体展示的短肽P1、P2具有天然ICAM-1的生物学活性。

AIM: To obtain bioactive ICAM-1-mimetic peptide. METHODS: Phages displaying P1 and P2 were prepared by phage am-plication and PEG precipitation. The binding between phage-displayed peptides and anti-ICAM-1 mAb 15. 2 was evaluated by sandwich ELISA and competitive ELISA. Bio-activities of P1 and P2 was detected by immunohistochemi-cal staining. RESULTS; Phage-displayed peptides P1 and P2 could specifically bind to mAb 15. 2, and the binding could be competitively inhibited by ICAM-1. Immunohisto-chemical staining showed that P1 and P2 could mimic the binding of ICAM-1 to its receptor LFA-1. CONCLUSION: Phage-displayed peptides P1 and P2 are bioactive just as native ICAM-1.