人绒毛膜促性腺激素对JEG-3细胞表达VEGF的影响

Effect of human chorionic gonadotrophin on vascular epithelial growth factor expression in JEG-3 cell line

目的 :揭示人绒毛膜促性腺激素 (hCG)对滋养层细胞表达血管内皮生长因子 (VEGF)的影响。方法 :以滋养层细胞来源的JEG - 3细胞系为研究对象 ,采用半定量逆转录多聚酶链反应 (RT -PCR)方法检测VEGFmRNA的表达。结果 :分别用 (0、50、50 0、5 0 0 0、2 5 0 0 0 )U/LhCG处理 48h后 ,JEG - 3细胞中VEGF的表达被诱导增高 ,且包括VEGF1 2 1 、VEGF1 6 5、VEGF1 89在内的VEGF亚型均受到诱导。另外 ,观察VEGF在 2 5 0 0 0U/LhCG处理的JEG - 3细胞中表达的时间变化 ,结果发现VEGF在JEG - 3细胞中的表达在经过短暂的诱导后略降低。结论 :hCG可能通过调节VEGF在滋养层细胞或滋养层细胞来源的绒癌细胞中的表达 ,进而影响细胞的增殖。

AIM: To uncover the effect of human chorionic gonadotrophin (hCG) on vascular epithelial growth factor (VEGF) expression in trophoblasts. METHODS: The semi-quantitative method of reversely transcription polymerase chain reaction (RT-PCR) was employed to investigate the expression of VEGF in trophoblast-derived JEG-3 cell line. RESULTS: VEGF 121 , VEGF 165 and VEGF 189 was shown to express in JEG-3 cells. After incubated with 0, 50, 500, 5 000 , 25 000 U/L hCG for 48 hours respectively, the expression of VEGF (including its 3 isoforms: VEGF 121 , VEGF 165 , VEGF 189 ) was induced gradually with the concentration of hCG increased. Furthermore, VEGF mRNA expression in JEG-3 cells incubated with 25 000 U/L hCG experienced a transient increase before a less significant decrease. CONCLUSION: The autocrine of VEGF might be involved in the effect of hCG on trophoblast or trophoblast-derived choriocarcinoma cell proliferation, migration and invasiveness.