栽培稻种间近等基因系杂种育性遗传及其基因定位

Hybrid Sterility Inheritance and Mapping of Sterile Genes among Near-Isogenic Lines Derived from Interspecific Hybrid Between Cultivated Rice Species Oryza sativa and O. glaberrima

用粳型亚洲栽培稻品种 WAB56- 1 0 4与来自种间回交组合 WAB56- 1 0 4 /CG1 4∥ WAB56- 1 0 4 ///WAB56- 1 0 4的3个栽培稻种间近等基因系杂交、回交 ,对 3个 BC1F1群体的花粉及小穗育性遗传研究表明 ,育性遗传符合单位点孢子体 -配子体互作模型 ,来自非洲栽培稻的不育基因在与相同位点的亚洲栽培稻等位基因互作时 ,导致携带亚洲栽培稻等位基因的雌雄配子败育 ,而形成花粉及小穗的半不育 ,但本研究的 3个组合中 ,并未导致所有携带亚洲栽培稻等位基因的雌配子完全败育 ,其作用介于配子消除与花粉灭杀之间。用微卫星标记对这 3个群体的育性基因定位表明 ,它们都位于第 6染色体短臂末端 ,但 WAB450 - 2、WAB450 - 8携带的不育位点与紧密连锁的微卫星标记 RM1 90、RM1 33共分离 ,且很可能与 S1等位 ,而WAB450 - 7的不育基因则与位于相邻区间的 RM2 53呈松散连锁 ,可能与 WAB450 - 2、WAB450 -

Three combinations of WAB56 104/WAB450 2∥WAB56 104, WAB56 104/WAB450 7∥WAB56 104, and WAB56 104/WAB450 8∥WAB56 104 were used to study inheritance of pollen and seed setting rate in Sanya, Hainan Province in the early season rice (March-July, 2001). WAB56 104 and CG14 were upland japonica rice (Oryza sativa) and O. glaberrima, respectively. WAB450 2, WAB450 7, and WAB450 8 were derived from interspecific BC 2 cross WAB56 104/CG14∥WAB56 104///WAB56 104. Results indicated that semi sterility of pollen and spikelet could be explained by one locus sporo gametophytic interaction model. However, elimination of female gametes was incomplete. Gene mapping with SSR markers showed that sterile gene in WAB450 2 and WAB450 8 was located on short arm of chromosome 6, cosegregated with closely linked SSR markers RM190 and RM133 respectively, which was high possible allelic to S 1 locus. But sterile gene in WAB450 7 was loosely linked to RM253, which indicated that the sterile gene locus in WAB450 7 might be different from that in WAB450 2 and WAB450 8.