野生大豆若干耐盐生理指标的研究

Studies on Some Physiological Characteristics of Salt-tolerance in Wild Soybeans (Glycine soja)

用NaCl溶液作为渗透胁迫剂模拟盐渍环境,对10个不同耐盐性的野生大豆材料分别用100mmol/LNa Cl和150mmol/LNaCl溶液胁迫.测定组织含水量、超氧自由基、MDA,SOD、POD、CAT酶活力,游离脯氨酸、可溶性糖等生理生化指标,并对比在NaCl渗透胁迫下的生理变化.表明SOD、POD和综合酶活力随着胁迫压力的增大而上升,脯氨酸、可溶性糖也逐渐积累,作为渗透调节物质脯氨酸和可溶性糖有相互补充的作用.聚类分析也表明,在2种胁迫下,在所测的生理指标中,酶活力在野生大豆的生理活动中具有相对重要的作用.

Ten accessionsof wild soybean (Glycine soja), withdifferent levels of salttolerance, were treated in 100 mmol/L and 150 mmol/L NaCl solutions for 48 h. A number of physiological characteristics of the wild soybean, including O·-2,SOD,POD,CAT,MDA,free proline ,and soluble sugar in the leaf of wild soybeans,were detected and compared between normal and NaCl stress.The results showed that the activity of enzymesascendsand osmotic adjustment solutes accumulated during salt stress. It seems that the free proline and soluble sugar were complemented each other. The clusteringanalysis of 10 accessions based on above 7 characteristics also indicated thatenzymes werethe most important inmany indexes inaccessing salt tolerance under thoseconcentration ofNaCl.