活跃传毒介体灰飞虱(Laodelphax striatellus)品系的杂交与选育

Screening of Active Vector Planthoppers (Laodelphax striatellus) by Selection and Cross Breeding

对水稻条纹叶枯病毒传播介体进行连续4个世代的杂交和严格的人工选育,获得活跃传毒的介体品系,其传毒率从F0群体的5.31%上升到25%,该介体品系经2年时间无人工选择压力的连续饲养,在间断选育的第5代,其传毒率明显下降,仅有14.75%.可见人工选择压对介体传毒力这一性状的稳定起着重要的作用,但对介体获毒没有影响;单头昆虫的DIBA技术使测定标本用量可以少至1/30头灰飞虱,带毒虫率从F0群体的12.09%上升到F4代的32.6%.

An active colony of Laodelphax striatellus, vector of the rice stripe virus was obtained by cross breeding and selecting for four successive generations and its transmitting ability was increased from 5.31% at F0generation to 25% in the fourth generation. When the colony was kept in a free selection pressure for two years, the transmitting ability was reduced to 14.75%. It is apparent,then,that the selection pressure could play an important role in maintaining vector transmission. However,no impact was found on insect acquisition feeding. It was still detectable by dot immunobinding assay (DIBA) that individual planthopper extraction was diluted to 1/30 and the incidence of insect acquisition rose from 12.09% in F0generation to 32.6% in the fourth generation.