阿司匹林对大鼠脑缺血再灌注损伤的影响

Effect of ASA on Brain I/R Injury in Rats

目的 :研究阿司匹林对脑缺血再灌注损伤的影响。方法 :实验分为假手术组、对照组 (10 %乙醇 )、ASA10mg/kg组、ASA4 0mg/kg组和ASA16 0mg/kg组。采用线栓法制备短暂性大脑中动脉缺血模型 (缺血 2h ,再灌注 2 2h) ,观察ASA对脑组织中中性白细胞浸润 ,NF -κB活性 ,IL - 1β、TNF -α和ICAM - 1的表达 ,以及脑梗死体积、神经功能缺损。结果 :ASA可抑制核因子NF -κB激活 ;抑制ICAM - 1、IL - 1β和TNF -α的表达 ;减少中性白细胞浸润 ;减小梗死体积积百分比 ,以ASA 10、4 0mg/kg组的作用更明显 ;对 5分制神经功能评分无影响。结论 :①ASA可减轻脑缺血再灌注损伤 ,小剂量的ASA作用效果较好 ;②脑缺血再灌注损伤过程中存在急性炎症反应 ,ASA可抑制脑缺血再灌注损伤过程中的炎症反应 ;③ASA减轻脑缺血再灌注损伤的机制可能与其抑制炎症反应有关 ,然而小剂量的ASA具有较好的疗效 。

Objective:To study the effect of ASA on brain I/R injury in rats. Methods: Sixty male SD rats were randomized in the sham operation,control,ASA 10 mg/kg,ASA 40 mg/kg and ASA 160 mg/kg groups. MCA occlusion was achieved by advancing a nylon filament into the internal carotid artery. After MCA occlusion for 2 hours and reperfusion for 22 hours,the neutrophil infiltration,the activity of NF-κB,the expression of TNF-α,IL-1β and ICAM-1 in brain tissue,the infarct volume and the neurologic deficit were studied. Results: The ASA inhibited the neutrophil infiltration,activity of NF-κB,the expression of ICAM-1,IL-1βand TNF-α,reduced the infarct volume(ASA10 and 40 mg/kg were more efficient),but yielded no effects on neurologic deficit scores. Conclusions: ①ASA reduced the brain I/R injury,which is more efficient with low dose. ②There was an acute inflammation in brain ischemic reperfusion,which could be inhibited by ASA. Such inhibition might give rise to a reduction of brain I/R injury. ③As the treatment efficacy with low dose ASA is more significant,it is suggested that the neuroprotection of ASA is related to the improvement of cerebral blood flow during brain ischemic reperfusion.