摘要
目的 :制备聚乙二醇 -超氧化物歧化酶结合物 (PEG -SOD)并比较天然SOD与PEG SOD的稳定性。方法 :采用氰脲酰氯法先对聚乙二醇 - 6 0 0 0 (PEG - 6 0 0 0 )进行活化 ,再以活化后的聚乙二醇对牛血Cu ,Zn SOD进行化学修饰 ,制得PEG SOD。将天然SOD与PEG SOD分别进行热稳定性、酸碱稳定性及蛋白酶稳定性的实验。结果 :PEG SOD的活性保留率在 70℃和 80℃的水浴中均比天然SOD高出 10 %～ 2 0 % ,在不同的 pH下 ,PEG SOD的活性保留率均比天然SOD高 10 %～ 4 0 % ,在胃蛋白酶和胰蛋白酶中PEG SOD的活性保留率均比天然SOD高9%～ 11%。结论 :PEG SOD比天然SOD在热稳定性、酸碱条件及抗酶解三方面的稳定性均有不同程度的提高。
Objective:To compare native SOD with PEG SOD(PEG-6000)in their stability.Methods: PEG SOD(PEG-6000) was obtained after Cu,Zn SOD in bovine blood was modified byPEG-6000 activated with cyanuric chloride method. Compare native bovine SOD with PEG SOD in stability by experiments of heat,acid,alkali and proteinase. Results: Conpared with native SOD,the activity remaining of PEG SOD was 10～20% higher at temperatures of 70 ℃ and 80 ℃,10～40% higher at different pH values and 9～11% higher under the effect of pepsin and trypsin. Conclusion: It was shown that PEG SOD was more stable than native SOD.
出处
《广州医学院学报》
2002年第3期16-18,共3页
Academic Journal of Guangzhou Medical College
关键词
超氧化物歧化酶
聚乙二醇
稳定性
superoxide dismutase
polyethylene glycol
stability
