正常早孕与流产患者蜕膜局部PRL、P、E_2水平及其受体mRNA表达水平的比较

Comparison of PRL,P,E_2 levels and Their Receptors mRNA Expression in Normal and Abortional Decidue

目的:比较正常组与流产组蜕膜PRL、P、E_2及其受体mRNA水平的差异,探讨其在早孕维持中的作用。方法:收集18例正常早孕人流与12例保胎失败刮宫患者的蜕膜组织,应用放免法测定蜕膜匀浆PRL、P、E_2水平,并行RT-PCR方法检测了相应激素受体(PRLR、PR、ER)mRNA表达的差异。结果:正常早孕组蜕膜匀浆PRL、P水平显著高于流产组(P<0.05),PRLR、PRmRNA表达正常早孕组亦显著高于流产组(P<0.05和0.01)。结论:蜕膜局部正常水平的PRL、P及其受体mRNA的正常表达对妊娠的维持起着重要的作用。

Objective: To compare the difference of PRL,P,E2 and their receptors mRNA in normal and abortion-al decidue so as to explore their effects on maintaining pregnancy. Methods: The decidue from 18 normal early pregnancy women who desired conducting induced abortion and decidue from 12 women who failed to prevent miscarriage were collected. RIA was carried out to measure the local hormone levels, and RT-PCR was used to detect the expression of PRLR,PR,ERmRNA. Results: The decidue PRL and P levels of normal early pregnancy group were significantly higher than those of miscarriage group (P<0. 05), the same as PRLR (P<0. 05) and PRmRNA (P<0. 01). Conclusion: The normal excretion of PRL,P and the normal expression of their receptors mRNA in decidue are important in maintaining early pregnancy.