新型耐酸性α-淀粉酶液态发酵条件的研究

Production of A Novel Acid-resistant α-amylase in Liquid Culture

通过基因工程技术导入了产生耐酸性α-淀粉酶和糖化酶的多考贝融合基因而获得的白曲霉基因工程菌株TR12作为试验菌株,分别在摇瓶条件下和2L通气发酵罐上进行耐酸性α-淀粉酶的液态发酵工艺条件的试验研究。正交试验结果表明,优化摇瓶发酵培养基配方A3B3C2D1,即玉米粉4%,玉米浆1.5%,黄豆粉2%,麸皮4%,摇瓶产酶活力81.69u/mL,2L发酵罐的产酶活力达到了83.6u/mL的水平。

With the transformant strain TR12, containing multiple copies of acidresistant α-amylase and glucoamylase genes from white mold Aspergillus Kawachii. The acidresistant α-amylase fermentation was studied in shaking flask and 2L ventilating fermenter,respectivly. Anoptimum culture medium A3B3C2D1 was obtained by crosstests, whichis corn flour 2%, corn slurry 1.5%, soybean flour 2%, and bran 4%. The acidresistant α-amylase activity reachs 81.69?u/mL in shaking flask,and 83.6?u/mL in 2L fermenter.