补锌对缺锌烫伤大鼠肠粘膜营养状态的影响

The effects of zinc supplements after burns on the nutritional state of gut mucosa in rats with zinc-deficient

目的 :研究补锌对缺锌烫伤大鼠肠粘膜营养状态的影响。 方法 :1月龄大鼠进食低锌饲料 (含锌量为 1.6μg/g) 1周 ,造成缺锌模型 ,2 0 %深二度烫伤后分别改饲三种不同含锌量饲料 (各为 1.6 μg/g、2 4 .7μg/g和 2 86 .9μg/g) ,同时设一组大鼠烫伤前后均进食正常含锌饲料 (2 4 .7μg/g)作对照组。伤后第 8天处死并留取标本。动物处死前注射长春新碱 ,以肠腺细胞增生率 (CCPR)、粘膜湿重、空肠与回肠的DNA、蛋白质含量反映肠粘膜的营养状态。 结果 :各补锌组空肠DNA含量及CCPR均显著高于缺锌组 ,回肠也存在类似的趋势。 结论

Objectives:To investigate the effects of zinc supplements on the nutritional state of gut mucosa after major burns. Methods:Weanling rats were fed zinc deficient chows (zinc content: 1.6 μg/g) for seven days to induce zinc deficient status. Then 20% TBSA deeper second degree scalds were made and the rats were divided into three groups fed on chows different in zinc contents. Zinc contents were 1.6 μg/g, 24.7 μg/g, 286.9 μg/g respectively. A group of rats fed normal zinc content (24.7 μg/g) diets before and after scalds was designed as control. Rats were killed on the eighth day after burns and certain sections of jejunum and ileum were resected. Vincristine was injected 6 hours before they were killed, which was supposed to block cell division in metaphase. DNA and protein contents as well as crypt cell production rate (CCPR) were calculated. Results: DNA contents and CCPR of jejunal mucosa were markedly improved in the group of zinc supplements. Protein contents were also improved to some extent. The same trend could be seen in ileac mucosa too. Conclusions: Zinc plays an important role on the nutritional states and proliferation of gut mucosal cells. To supplement zinc after major burns in rats with zinc deficent could help to repair the injured intestinal mucosa.