生殖系多种病原体感染与男性不育的关系

RELATIONSHIP BETWEEN POLYPATHOGEN INFECTION OF GENITOURINARY TRACT AND MALE INFERTILITY

目的 探讨男性不育患者弓形体、解脲支原体、沙眼衣原体感染情况和抗精子抗体在男性不育中的作用。 方法 应用多聚酶链反应 (PCR)技术分别对 112例男性不育病人和 6 2例正常生育者的精液进行精浆弓形体 DNA、解脲支原体和沙眼衣原体 DNA的检测 ,用金标免疫斑点试验检测精浆抗精子抗体 (As Ab)。 结果 男性不育组弓形体阳性 2 0例 (17.86 % ) ,解脲支原体阳性 35例 (31.2 5 % ) ,沙眼衣原体阳性 18例 (16 .0 7% ) ,抗精子抗体阳性 33例 (2 9.4 6 % )。3种病原体感染率与抗精子抗体的阳性率均明显高于正常对照组 (P<0 .0 1) ,且多种病原体感染患者抗精子抗体的阳性率明显高于单项感染者。 结论 男性不育患者多种病原体感染后 ,导致抗精子抗体的产生是男性不育的重要免疫因素。

Objective To probe the condition of Toxoplasma gondii (TOX), Ureaplasma urealyticum (UU), Chlamydia trachomatis (CT) infection of genitourinary tract and the role of antisperm antibody(AsAb) in male infertility. Methods The DNA of TOX, UU and CT in serous sperm were detected by polymerase chain reaction(PCR) from 112 male cases of infertile and 62 cases normal fertility respectively, AsAb in serous sperm were detected by the Dot immunogold test. Results There were 20 cases of positive TOX in the group of male infertility (17.86%), 35 cases of positive UU (31.25%), 18 cases of positive CT (16.07%), 33 cases of positive AsAb (29.46%). The infection rate of the three pathogens and the positive rate of AsAb were significantly higher than normal group of contrast ( P <0.01) respectively, and the positive rate of AsAb in polypathogen infection patients was significantly higher than that in single infection. Conclusion AsAb was the important immune factor causing infertility after polypathogen infection in male infertility.