β淀粉样蛋白对大鼠额叶和海马细胞外液单胺类递质的影响

Effects on Amyloid-β protein in extracellular monamine neurotransmission of frontal cortex and hippocampus in rats

目的 探讨 β淀粉样蛋白 (Aβ)与脑内单胺类神经递质的关系。 方法 将雄性SD大鼠 32只 ,随机分为模型组、尼莫地平治疗组、参麦治疗组和对照组 ,每组 8只。制作大鼠Alzheimer病模型并进行微透析收细胞外液。高效液相色谱电化学检测器检测样本单胺类递质。结果 发现模型组额叶NE、DA、5 HT均降低 ,低于对照组 ;尼莫地平治疗后 ,单胺类递质浓度恢复正常对照水平 ,海马区DA甚至高于对照组 ;参麦治疗后 ,结果类似尼莫地平 ,两组治疗效果无显著性差别 ,只是不同脑区 3种递质升高水平不同。结论 Aβ的神经毒作用与单胺系统功能低下有关 ,Aβ对单胺系统的抑制可能是包括Ca2 +

Objective To investigate the relationship between the neurotoxicity of Aβ and monoamine neurotransmissions in brain.Methods 32 male SD rats were divided into four groups: The model group, Nimodipine treatment group, Shenmai treatment group and the control group,there are 8 rats in each group.Under the stereotaxis Aβ was injected into NBM of rats to establish AD model, The extracellular monoamine neurotransmissions were detected by microdialysis in vivo with high performance liquid chromatography.Results The contents of frontal lobe NE,DA,5 HT in the model group were lower than those in the control group, which recovered to normal level,DA in hippocampus was higher than the control group;after the treatment of Shenmai,the result was similar to Nimodipine group.There was no difference between the two treatment groups.The rising levels of three kinds of transmitters in different brain area were different.Conclusion Neurotoxicity of Aβ might relate to dysfunction in monoamine system. Aβ on monamine system of inhibition was shown through multiple pathways, including the loss of Ca 2+ homeostasis.