巨结肠及类缘病诊断和治疗的临床研究

Application of NSE and S-100 protein immunohistochemical markersin diagnosis of allied HD

目的 探讨巨结肠类缘病的病理形态学特征 ,提高其诊断率。方法 对 97例行巨结肠根治术后的病变肠段进行常规HE染色 ,观察肠神经元及神经节细胞形态和数量的变化并与正常组进行比较。并对 2 1例采用NSE和S -10 0蛋白免疫组化染色。结果 巨结肠类缘病 (alliedHD)标本的神经元与神经节细胞数与正常组及巨结肠症 (HD)比较差异有显著性 (P <0 .0 1)。结论 巨结肠类缘病的诊断主要根据病理特征而定 ,在HE染色基础上结合NSE及S -10

Objective To study the pathological characteristics of allied Hirschsprung's disease (allied HD) by using NSE and S-100 protein immunohistochemical markers techniques,improve the early diagnosis rate. Methods 97 cases with HD were included in this study.The morphological and numeral change of the neuron and ganglion cell of the lesions was observed and compared with the normal controls.NSE and S-100 protein immunohistochemical markers were performed in 21 cases.Result The number of the neuron and ganglion cell of allied HD was statistically significantly higher than those in the normal control or HD group (P<0.01).Conclusions The diagnosis of allied HD is depended mainly on its pathologic characteristics.Introducing of NSE and S-100 protein immunohistochemical markers staining combined with usual HE stain would be greatly helpful in making a definite diagnosis.