陕西省HIV-1分子流行病学研究

Study of the Molecular Epidemiology of H1V-1 in Shaanxi Province

目的研究陕西HIV-1流行亚型及相互关系,分析流行时间及传播途径,为陕西省HIV的预防、控制及感染者治疗提供有力的依据。方法用PCR对15份陕西HIV-1感染者外周血单核细胞(PBMCs)样本进行扩增,获得HIV-1 env基因的核酸片段,对其C2-V3及邻区350-450个核苷酸序列进行分析,所得结果与HIV国际标准株及周边省份流行株进行比较,分析共享序列及突变序列,制作系统树,计算离散率。结果 陕西流行的B、E、C亚型内基因离散率分别为3.0％,1.7％和2.3％。10个B亚型毒株与泰国、我国云南、新疆、河南和四川流行的B亚型代表株基因序列接近;2个C亚型毒株我国新疆流行的C亚型代表株接近;3个E亚型毒株与河南流行的E亚型代表株接近。结论 陕西HIV-1流行株主要为B、E、C亚型,三亚型毒株在陕西的流行时间分别在2.5～3年、2～2.5和1.5～2年之间,传播来源可能为国外或周边省份传入。

Objective To study the molecular epidemiology of HIV-1 in Shaanxi Province,analysize possible epidemic time period and transmission routines,and provide base data for provention and treatment of HIV infection. Methods HIV-1 env gene were amplified by PCR from peripheral blood mononuclear cells (PBMCs) obtained from 15 HIV-1 infectors from Shannxi. The C2-V3 region (350- 450 bp) of them were sepuenced and compared with common epidemic strains found in other countries and other neighbor provinces around Shaanxi Province. Results Sequence analysis showed that there were three HIV-1 subtypes-B,E and C in Shannxi, with 3.0 % , 1.7 % and 2.3% gene divergence inside each subtype. The 10 subtype B strains were closely related to those found in Thailand and Yunnan,Xinjiang,Henan,Sichuan province of China. The 3 subtype E and 2 subtype C strains were closest to those found in Henan as well as Xinjiang. Conclusion HIV-1B, E,C subtypes were main epidemic subtypes in Shaanxi Province. Possible epidemic time period were 2.5-3years,2-2. 5years and 1.5-2years respectively. It was likely that they came from abroad or neighbor provinces around Shaanxi Province.