肺炎衣原体肺部感染的超微病理研究

Ultrastructural lung pathology of experimental Chlamydia pneumoniae pneumonitis in mice

目的 :通过研究小鼠肺组织超微病理改变 ,对肺炎衣原体肺部感染的发病机制进行初步探讨。 方法 :以肺炎衣原体鼻内或静脉接种Icr小鼠 ,在不同时点 (1、3、7、14、2 1、2 8和 6 0天内 )处死动物 ,以透射电镜观察小鼠肺炎衣原体肺炎急性期肺组织超微病理改变。 结果 :小鼠吸入肺炎衣原体后第 3天在肺间质、支气管腔和肺泡腔可见明显多形核白细胞浸润 ,病原体感染肺泡上皮细胞 ,形成各种发育阶段的肺炎衣原体包涵体。 7天后在支气管及肺泡间质中单核细胞浸润呈上升趋势 ,肺泡隔中见Ⅱ型上皮细胞、成纤维细胞增生 ,但未再见到肺炎衣原体的包涵体。静脉接种组引起上述类似改变 ,但程度轻 ,时间短 ,未见包涵体形成。 结论 :通过透射电镜观察小鼠肺组织超微病理改变 ,对肺炎衣原体肺炎急性期的诊断提供依据。本组资料还显示 。

Objectives:To investigate the ultrastructural pathogenesis of Chlamydia pneumoniae (C.pneumoniae) pneumonitis by using transmission electron microscope. Methods:The Icr mice were inoculated with C.pneumoniae, strain CWL 029, by the intranasal or intravenous routes. After a single inoculation, mice were killed on the 1st, 3rd, 7th, 14th, 21st, 28th and 60th day separately. Lung specimens were removed of the acute stage of C.pneumoniae pneumonitis and viewed in a transmission electron microscope. Results:In the intranasal inoculation of mice, the inflammatory infiltration was predominantly polymorphonuclear leukocytes on day 3. By day 7, mononuclear cells were most prominent in the infiltration. On day 3, chlamydial inclusions were founded in the alveolar epithelial cells. Inclusions were difficult to find after day 7. After iv inoculation, a similarly changes were seen but inclusions were difficult to find. Conclusions:These ultrastructural observations are beneficial to the diagnosis of the acute stage of C.pneumoniae pneumonitis in the mice, and the data in this series also showed that the pathogenesis of infection in intranasal inoculation group was more serious than that of iv inoculation group.