双丁酰环磷酸腺苷对人精子运动功能的影响

Influence of Dibutyryl Cyclic Adenosine Monophosphate on Motility of Sperm

目的 :通过研究双丁酰环磷酸腺苷 (dbcAMP)对体外人精子的运动功能有无影响 ,了解环磷酸腺苷 /蛋白激酶A(cAMP/PKA)信号传导通道是否参与人精子运动功能的调节。 方法 :10例健康生育男性手淫获得并经上游优化处理的精子与不同浓度的dbcAMP一起孵育 2 0、30、6 0min后 ,采用计算机辅助的精液分析系统 (CASA)检测精子的运动参数。 结果 :dbcAMP在体外能显著提高人精子的活率及前向性运动百分率 ,并且随浓度的增加 ,这种作用显著增强 ,而对精子的形态及直线速度 (VSL)和曲线速度 (VCL)无明显影响。 结论

Objectives: To investigate the influence of cAMP/PKA signal transcduction on human sperm motility, and to study the effect of dibutyryl cyclic adenosine monophosphate dibutyryl cyclic adenosine monophosphate (dbcAMP) on human sperm motility in vitro . Methods: Sperm aseptically obtained by masturbation and prepared by swim up technique from 10 healthy fertile men were incubated with different concentrations of dbcAMP. Measurement of mobility were carried out at 20, 30, and 60 min in all specimens. Results: The sperm treated with dbcAMP showed a significant increase in sperm progressive motility and the percentage of motile cells. The effect seemed enhanced with the increasing of dbcAMP concentration. VSL and VCL were not affected by dbcAMP. Conclusions: dbcAMP can activate the mobility of human sperm in vitro .