精索静脉曲张大鼠生精细胞凋亡的实验研究

The Apoptosis in Damaged Testicular Tissue Caused by Varicocele

目的 :探讨外科所致的精索静脉曲张对大鼠生精细胞凋亡的影响。 方法 :采用成年雄性Wistar大鼠复制左精索静脉曲张模型 ;用原位末端脱氧核苷酸转移酶标记法 (TUNEL)检测睾丸生精细胞凋亡。 结果 :实验组的细胞凋亡率显著高于假手术对照组 (P <0 .0 5 )。 结论 :外科所致的精索静脉曲张大鼠睾丸生长减缓 ,生精细胞凋亡增加 ,这可能是其影响生育能力的机制之一。

Objectives: To investigate the effects of experimental varicocele on apoptosis of spermatogenic cells in rats. Methods: Experimental varicocele was induced by partial ligation of the left renal vein in Wistar rat. Apoptosis cells were detected by in situ terminal deoxynucleotityl transferase mediated dTUP nick end labeling(TUNEL) technique. Results: There was certain proportion of apoptosis cells in spermatogenic cells in control group rats.The incidence of apoptosis increased remarkably in experimental group, the testicular growth was slower than that in control group. Conclusions: The apoptosis of spermatogenic cells caused by varicocele may play an important role in leading to subfertility.