胃癌组织中凋亡相关基因survivin表达及其与p53、k-ras表达的相关关系

Expression of survivin gene and its relationship with p53,k-ras proteins in human gastric carcinoma

目的 探讨凋亡相关基因survivin在胃癌组织中的表达 ,及其与 p5 3、k ras表达的相关性。方法 应用免疫组织化学链酶亲和素 生物素 过氧化物酶复合物法 (SABC法 ) ,检测 2 0例正常胃粘膜组织及 12 0例胃癌组织中的survivin、p5 3和k ras的表达。结果 2 0例正常胃粘膜组织中survivin呈阴性表达 ,而 12 0例胃癌组织中 92例呈阳性表达 ,阳性率为 76 7% ,差异有显著性 (P <0 0 5 )。survivin基因表达与胃癌组织分化程度、临床分期及淋巴结转移呈正相关。p5 3与survivin基因表达显著相关 ,k ras蛋白表达与survivin基因表达无明显相关性 (P >0 0 5 )。结论 (1)survivin基因在胃癌组织中表达上调 ,提示该基因对胃癌发生发展起重要的作用。survivin基因的异常表达提示胃癌预后不良。 (2 )抑癌基因 p5 3的失活与survivin基因的表达可能在胃腺上皮细胞癌变中起协同作用 ,survivin基因与k

Objective To study the expression of survivin and its relationship with the expression of p53,k-ras in gastric carcinoma.Methods Immunohistochemical assay was performed to detect the expression of p53,k-ras proteins in 120 gastric carcinoma samples and 20 normal gastric mucous tissue samples.Results Expression of survivin gene was detected in a significantly great proportion of gastric carcinoma(76 7%),and wasnt found in normal gastric mucous tissues( P< 0 05).There was a relationship between survivin gene expression with differentiation,clinical stages and lymph node metastases.The expression of survivin gene was correlated with p53,but not with k-ras expression.Conclusion (1)The up-regulation expression of survivin gene in gastric carcinoma suggested that survivin may play a role in the pathway of carcinogenesis.Survivin over-expression provides non-prognostic information of gastric carcinoma.(2)Survivin and de-activation of antioncogene p53 might play synergetic roles in the process of carcinogenesis of gastric carcinoma.But survivin and k-ras may be independently involved in the pathogenesis of gastric carcinoma.