人FADD基因诱导粘液表皮样癌细胞凋亡的研究

EXPERIMENTAL STUDY ON APOPTOSIS INDUCTION OF FADD GENE IN MUCOEPIDERMOID CARCINOMA CELLS

目的 观察人FADD基因对涎腺粘液表皮样癌细胞 (MEC - 1)的诱导凋亡作用。方法 通过反转录PCR获取人FADD基因 ,将基因克隆入绿色荧光蛋白真核表达载体pIRES2 -EGFP中 ,阳离子脂质体法转染粘液表皮样癌细胞 ,通过细胞记数、荧光显微镜及电镜观察、流式细胞仪等检测被转染细胞的生长及其凋亡状况。结果 与对照组相比 ,在转染 1～ 3天内 ,FADD基因的表达导致粘液表皮样癌细胞存活数目减少 ,大量细胞发生凋亡。结论 人FADD基因可以有效地诱导转染的粘液表皮样癌细胞凋亡。

Objective To investigate the induction of salivary gland mucoepidermoid carcinoma cells to apoptosis by the expression of FADD gene. Methods RT-PCR and recombinant PCR were used to amplify human FADD gene. Following cloning into green fluorescent protein expression vector pIRES2-EGFP and then transfecting mucoepidermoid carcinoma cells, we evaluated the growth condition and apoptosis of these cells by cell counting, fluorescent microscope photographs, electron microscope photographs, as well as flow cytometer assays. Results We successfully obtained human FADD gene. Compared with the control group, the expression of FADD gene caused the decrease of living cell numbers and massive occurrence of apoptosis. Conclusion FADD gene can effectively induce mucoepidermoid carcinoma cells to apoptosis.