丹仙康骨胶囊对培养成骨细胞增殖分化的影响

Effects of Danxian Kanggu Capsule on Proliferation and Differentiation of Cultured Osteoblasts in Vitro

目的 :为了解补肾活血中药丹仙康骨胶囊对体外培养成骨细胞的作用。方法 :应用MTT ,对硝基苯磷酸盐法及骨钙素含量放免测定法 ,观察丹仙康骨胶囊对成骨细胞增殖与分化作用的影响。结果 :丹仙康骨胶囊刺激成骨细胞增殖 ,提高ALP活性及骨钙素含量。结论

Objective :To investigate the effects of Danxian Kanggu(DXKG)capsule extracted from traditional Chinese medicine on cultured osteoblasts in vitro. Methods :MTT,PNPP and BGP content radioimmunoassay were used to observe the proliferation,the activity of ALP and the BGP content of osteoblasts cultured in vitro. Results :It was found that DXKG capsule had the effects on stimulating the proliferation,ALP activity and the BGP content of cultured osteoblasts in different concentration. Conclusion :DXKG capsule has effects on stimulating the proliferation and the differentiation,and increasing the activity of ALP and the BGP content of cultured osteoblasts in vitro.