ELISA检测干扰素抗体及临床意义

Detection of the antibody to IFNα-2b by ELISA and its clinical significance

目的 了解干扰素抗体对干扰素疗效的影响。方法 用ELISA检测 6 4例慢性乙型肝炎 (CHB)患者干扰素治疗前后血清的干扰素抗体 ,同时用荧光定量PCR技术检测干扰素治疗前后血清HBVDNA的含量。结果 6 4例接受干扰素治疗的CHB患者中的 2 8例患者检出干扰素抗体 (43 8% ) ,治疗前后的血清HBVDNA含量在干扰素抗体阳性组无显著性差异 (P >0 0 5 ) ,在干扰素抗体阴性组有显著性差异 (P <0 0 5 )。 2 8例干扰素抗体阳性者治疗结束时 ,2例 (7 14 % )患者血清中HBVDNA被清除 ,36例干扰素抗体阴性者在治疗结束时 ,12例 (33 33% )患者血清中HBVDNA被清除 ,二者比较P <0 0 5。结论干扰素抗体能影响干扰素的疗效 。

Objective To study the influence of antibody to IFNα 2b on the therapeutic effect of IFAα 2b.Methods The antibody to IFN in the sera from 64 patients with chronic hepatitis B was detected by ELISA.At the same time,HBV DNA in sera was quantitated at both beginning and end of treatment by a quantitative PCR assay which was based on Amplisensor assay.Results The antibody against IFN was found in 28 of 64 patients(43.8%) who had been treated with IFNα 2b.The contents of HBV DNA in sera at the beginning and the end of therapy were of no statistical difference in the IFN antiboday positive patients ( P >0.05).At the end of IFN therapy,HBV DNA was cleared away in 2 of 28(7.14%)of antibody positive patients and in 12 of 36 (33.33%)of antibody negative patients( P <0 05).Conclusion It is indicated that antibody to IFAα 2b may affect the response to IFN therapy.The detection of antibody to IFNα 2b is one of the methods that predict the therapeutic effect of IFN.