树状DNA杂交技术在HCV检测中的应用

The application of DDH technology in HCV detection

目的 建立一种敏感性、特异性、重复性较好的 ,适于病原体群体筛查的检验方法。方法 通过RT PCR DDH ,RT nested PCR和HCVRNA DDH 3种方法检测HCV核酸 ,推断RT PCR DDH技术检测病毒核酸的特异性、敏感性和稳定性。结果 4 7份ELISA检测HCV抗体阳性血清标本 ,RT nested PCR检出阳性标本 33例 (70 .2 1% ) ,RT PCR DDH检出阳性标本 39例 (82 .98% )。结论 RT PCR DDH技术在逆转录病毒核酸检测的应用中具有较好的特异性、敏感性和稳定性 ,而且成本较低 ,操作安全简便 。

Objective To set up a more sensitive,specific,duplicative method which should adapt to screen pathogenic microorganism in population.Methods By RT PCR DDH,RT nested PCR,RNA DDH,we detect the nucleic acid of HCV and deduce the specificity,sensitivity and stability of RT PCR DDH in viral nucleic acid detection.Result In 47 serum samples which were HCV positive in ELISA,and 33(70.21%) were positive in RT nested PCR,39(82.98%) were positive in RT PCR DDH.Conclusion RT PCR DDH has higher specificity,sensitivity and stability in retroviral detection.Moreover,low cost and easy,safe to operate make it is suitable for the test of pathogenic microorganisam in middle and small laboratories and primary hospitals.