Study on the metastable state of dilution of spectinomycin

采用诱导期法和直接法研究了大观霉素溶析结晶的介稳态.新相形成由多核机理控制.由多核机理得到两个操作温度下溶析结晶的介稳区.实验考察了丙酮浓度和温度对溶析结晶介稳态的影响.随着丙酮浓度的增大或温度的降低,诱导期延长.介稳区宽度随溶解度的减小而变宽.大观霉素初级成核的过饱和度水平较高,属于第Ⅰ类物系.根据经典成核理论估算了大观霉素成核参数.这些实验结果对大观霉素溶析结晶工业生产的控制具有重要意义.

The metastable state of dilution of spectinomycin has been studied by both induction period method and direct method. The polynuclear mechanism of losing metastablility has been disclosed. The metastable zone for dilution of spectinomycin was presented. The influence of acetone concentraion and temperature on the metastability has been investigated. The induction period elongated as acetone concentraion increased and temperature decreasd. The metastable zone width was broadened with the decreasement of solubility. High supersaturation level for nucleation indicated that spectinomycin belongs to class I system. On the basis of classical nucleation theory the fundamental nucleation parameters for dilution have also been estimated. All the results were important for the control of industrial dilution of spectinomycin.