嗜卷书虱和嗜虫书虱酯酶性质的比较研究

Comparison of the Activity of Carboxylesterase andAcetylcholinesterase in Liposcelis bostrychophila and L. entomophila

对嗜卷书虱 L iposcelis bostrychophila和嗜虫书虱 L . entomophila羧酸酯酶及乙酰胆碱酯酶(ACh E)的生物化学性质进行了初步研究。结果表明 :嗜虫书虱的羧酸酯酶活性明显高于嗜卷书虱 ,嗜卷书虱和嗜虫书虱羧酸酯酶对底物 α- NA的 Km 值分别为 0 .6 6 5 7、0 .2 85 7mmol/L,Vmax值分别为 1.6 72 2和 2 .4 6 31mmol· (mg· 30 min) -1。嗜卷书虱和嗜虫书虱乙酰胆碱酯酶的活性差异不显著 ,嗜虫书虱略高于嗜卷书虱。通过聚丙烯酰胺凝胶电泳 ,检测出 8条嗜卷书虱、7条嗜虫书虱酯酶同工酶谱带 。

The characteristics of carboxylesterase (CarE) and acetylcholinesterase (AChE) were investigated in Liposcelis bostrychophila and L. entomophila . The activity of CarE in L. entomophila was significantly higher than that in L. bostrychophila . The comparison of Michaelis constants ( K m) of CarE between L. entomophila and L. bostrychophila showed that the affinity of the former to substrate α NA was much stronger than that of the latter. The activity of AChE in the two insects was similar. Totals of 8 and 7 esterase bands were detected on PAGE for L. bostrychophila and L. entomophila , respectively. And the esterase zymogram of L. bostrychophila was wider than that of L. entomophila.